Abstract: Preservation technology during transportation is a key link in Agaricus bisporus industry. In order to choose the transportation route of fresh mushrooms more reasonably and adjust the real-time traffic information, an intelligent navigation strategy combined with the Beidou satellite navigation system is proposed. Firstly, the storage law of bis -mushroom is analyzed, and the realtime road condition is combined with the monitoring of the Beidou satellite navigation system. By using the clustering algorithm, a navigation strategy is made to select the most ideal transportation route intelligently. Finally, the feasibility of the strategy is verified by simulation.