Abstract: The composition and operation principle of the tail mark positioning system of  GNSS independently developed by China University of Petroleum are described in detail.Then it introduces the application of self-developed tail positioning system in marine streamer operation, which including design of its core hardware and software and main workflow. Finally, the radio transmission distance and stability and positioning accuracy are tested by land-based simulation experiments. The test results show that the tail positioning system can continuously and stably transmit data and can achieve centimeter-level relative positioning accuracy, which can meet the requirements of navigation and positioning for offshore towing cable operation in actual production.