Abstract: Global Navigation Satellite System is widely used because of its wide coverage and low cost. However, due to the use of MEO,IGSO and GEO satellites, the signal landing power is low and susceptible to interference. Therefore, to ensure the normal use of GNSS, it is necessary to carry out research on the detection and positioning of navigation interference sources. Aiming at the defects of low positioning accuracy, weak maneuverability, long positioning time, and high dependence on the environment, this paper proposes a navigation interference source detection and positioning system based on UAV. The design and implementation method is to realize the detection and positioning the navigation interference sources by carrying direction finding loads on unmanned aerial vehicles. The system has the advantages of long working distance, large coverage area and strong maneuverability.