Abstract: To ensure the normal application of the Global Navigation Satellite System (GNSS), monitoring and localization of GNSS interference radiation sources are required. This paper presents the design of a radiation source positioning system architecture. By designing the system functions, architecture, grid monitoring equipment and workflows, high-precision time synchronization and reliable time difference measurements are achieved using BeiDou/GPS timing + high-stability crystal oscillators and a generalized weighted time delay estimation algorithm, which ensures the accuracy of time difference of arrival (TDOA) localization. The effective location of radiation source is realized through system application test.