Abstract: At present, the research on the detection of navigation interference sources based on unmanned aerial vehicles is mainly focused on the theoretical research and simulation implementation stage. In this paper, we designed a Global Navigation Satellite System(GNSS) interference source direction finding and positioning system based on a six-rotor unmanned aerial vehicle (UAV) platform, and it was tested in a certain field on the positioning accuracy of the interference source. The system is mainly composed of a six-rotor unmanned aerial vehicle, an interference source monitoring direction finding load, and a ground station. After the trial production of the system is completed, we selected an open environment to verify the positioning accuracy of the interference source system for different type of tnterference with different power.